Receptor-Coupled Phosphoinositide Hydrolysis in Human Retinal Pigment Epithelium
نویسندگان
چکیده
منابع مشابه
Receptor-coupled phosphoinositide hydrolysis in human retinal pigment epithelium.
Carbachol and histamine stimulated phosphoinositide (PPI) hydrolysis in cultured human retinal pigment epithelium (RPE), as reflected by an accumulation of 3H-inositol phosphates in the presence of 10 mM Li+. Carbachol increased PPI hydrolysis to greater than 600% of basal with an EC50 of 60 microM; stimulation was linear up to 60 min. This activation likely occurred via the M3 muscarinic choli...
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PURPOSE Human retina and retinal pigment epithelium (RPE) express a relatively abundant mRNA that encodes an extraneous splice isoform of the RPE retinal G protein-coupled receptor (RGR) opsin. In this study, we investigate this exon-skipping RGR splice isoform (RGR-d) in separated neural retina and RPE cells of human donors of various ages. METHODS We used mass spectrometry, sensitive wester...
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ژورنال
عنوان ژورنال: Journal of Neurochemistry
سال: 1991
ISSN: 0022-3042,1471-4159
DOI: 10.1111/j.1471-4159.1991.tb03471.x